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Western blot analysis of extracts of HepG2 cells, using DIABLO antibody (13-302).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HepG2 cells, using DIABLO antibody (13-302).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

DIABLO Antibody

CATALOG NUMBER: 13-302

Clonality:
Polyclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide of human DIABLO
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
Flow: 1:20 - 1:50
Positive Control 1:
HepG2
Predicted Molecular Weight:
Observed: 22kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DIABLO
Additional Names:
DIABLO, SMAC, DFNA64
Ncbi Gene Id Number:
56616
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-302

List Size:
100 uL

List Price:

$365.00

Out of stock

Datasheet
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