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Antibody used in WB on Human Jurkat 0.3125 ug/ml.
Antibody used in WB on Human Jurkat 0.3125 ug/ml.

DIABLO Antibody

CATALOG NUMBER: 31-015

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DIABLO.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DIABLO antibody can be used for detection of DIABLO by ELISA at 1:312500. DIABLO antibody can be used for detection of DIABLO by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
21 kDa, 27 kDa, 22 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DIABLO antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DIABLO
Additional Names:
DIABLO, SMAC, DFNA64
Protein Accession Number:
NP_620308
Protein Gi Number:
21070978
Ncbi Gene Id Number:
56616
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DIABLO is an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively.
Background Reference 1:
Samuel, T., et al., (2006) J. Biol. Chem. 281 (2), 1080-1090.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-015

List Size:
100 ul

List Price:

$473.00

Datasheet
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