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Antibody is used in Western blot to detect DGKI in mouse cerebellum tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis in A2058 cell line lysates (35ug/lane).
Antibody is used in Western blot to detect DGKI in mouse cerebellum tissue lysate.

Diacylglycerol kinase iota Antibody

CATALOG NUMBER: 63-406

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Diacylglycerol kinase iota (DGKI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 890-920 amino acids from the C-terminal region of human Diacylglycerol kinase iota (DGKI).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
117 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DGKI
Additional Names:
Diacylglycerol kinase iota, DAG kinase iota, Diglyceride kinase iota, DGK-iota, DGKI
Protein Accession Number:
O75912
Ncbi Gene Id Number:
9162
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DGKI a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.
Background Reference 1:
Ding, L., et al., J. Biol. Chem. 273(49):32746-32752 (1998).
Background Reference 2:
Bowne, S.J., et al., Mol. Vis. 6, 6-9 (2000).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-406

List Size:
400 ul

List Price:

$495.00

Datasheet
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