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42-285 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-285 (0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DIAPH1 Antibody

CATALOG NUMBER: 42-285

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-QTKTSKAKKDQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141kDa according to Human NP_005210.3 and 139kDa according to Mouse NP_031884.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DIAPH1
Additional Names:
DIAPH1, diaphanous homolog 1 (Drosophila), DFNA1, DIA1, DRF1, FLJ25265, LFHL1, hDIA1, diaphanous 1, diaphanous-1, diaphanous-related formin 1
Protein Accession Number:
NP_005210.3,NP_001073280.1
Protein Gi Number:
119395758
Ncbi Gene Id Number:
1729
Background Reference 1:
Higashi T, Ikeda T, Shirakawa R, Kondo H, Kawato M, Horiguchi M, Okuda T, Okawa K, Fukai S, Nureki O, Kita T, Horiuchi H Biochemical characterization of the Rho GTPase-regulated actin assembly by diaphanous-related formins, mDia1 and Daam1, in platelets J Biol Chem. 2008 Mar 28;283(13):8746-55

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-285

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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