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Western blot analysis of DIAPH2 Antibody in 293, MDA-MB435 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with DIAPH2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DIAPH2 Antibody in 293, MDA-MB435 cell line lysates (35ug/lane)

DIAPH2 Antibody

CATALOG NUMBER: 63-930

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DIAPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 870-899 amino acids from the Central region of human DIAPH2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
126 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DIAPH2
Additional Names:
Protein diaphanous homolog 2, Diaphanous-related formin-2, DRF2, DIAPH2, DIA
Protein Accession Number:
O60879
Ncbi Gene Id Number:
1730
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DIAPH2 belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this protein have been linked to premature ovarian failure 2.
Background Reference 1:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 2:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 3:
Yasuda, S., et al. Nature 428(6984):767-771(2004)
Background Reference 4:
Gasman, S., et al. Nat. Cell Biol. 5(3):195-204(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-930

List Size:
400 ul

List Price:

$495.00

Datasheet
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