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DIAPH3 Antibody

CATALOG NUMBER: 45-478

Clonality:
Polyclonal
Tested Applications:
E
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-RTPMPKDVRQS
Conjugate:
Unconjugated
Tested Applications:
E
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 50kDa band in human bone marrow, liver and lymph node lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to NP_001035982.1 and 98.1kDa according to NP_112194.2. The ~50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognise both reported isoforms (NP_001035982.1 and NP_112194.2).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Ncbi Official Symbol:
DIAPH3
Additional Names:
DIAPH3, diaphanous homolog 3 (Drosophila), RP11-26P21.1, DKFZP434C0931, DKFZp686A13178, DRF3, FLJ34705, diap3, diaphanous homolog 3, diaphanous-related formin 3
Protein Accession Number:
NP_001035982.1, NP_112194.2
Protein Gi Number:
110225351
Ncbi Gene Id Number:
81624
Background Reference 1:
Yang C, Czech L, Gerboth S, Kojima S, Scita G, Svitkina T. Novel roles of formin mDia2 in lamellipodia and filopodia formation in motilecells. PLoS Biol. 2007 Nov;5(11):e317.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-478

List Size:
0.1 mg

List Price:

$325.00

Out of stock

Datasheet
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