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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

DIAPH3 Antibody

CATALOG NUMBER: 58-585

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DIAPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1090 amino acids from the C-terminal region of human DIAPH3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
137 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DIAPH3
Additional Names:
Protein diaphanous homolog 3, Diaphanous-related formin-3, DRF3, MDia2, DIAPH3, DIAP3
Protein Accession Number:
Q9NSV4
Protein Gi Number:
158520000
Ncbi Gene Id Number:
81624
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).
Background Reference 1:
DeWard, A.D., et al. J. Biol. Chem. 284(30):20061-20069(2009)
Background Reference 2:
Block, J., et al.Microsc 231(3):506-517(2008)
Background Reference 3:
Beli, P., et al. Nat. Cell Biol. 10(7):849-857(2008)
Background Reference 4:
Yang, C., et al. PLoS Biol.(11), E317 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-585

List Size:
400 ul

List Price:

$495.00

Datasheet
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