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45-479 staining (0.1ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-479 (1ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
45-479 staining (0.1ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Dicarbonyl Reductase Antibody

CATALOG NUMBER: 45-479

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: GSTLPVEGGFWAC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in human kidney, liver and testes lysates (calculated MW of 25.9kDa according to NP_057370.1). Recommended for use at 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded human kidney, stains membrane / cytoplasm in epithelial cells of renal glomeruli and tubules. Recommended concentration, 1-2ug/ml.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DCXR
Additional Names:
DCXR, dicarbonyl/L-xylulose reductase, DCR, HCRII, KIDCR, P34H, carbonyl reductase, carbonyl reductase II, kidney dicarbonyl reductase, HCR2, SDR20C1, human carbonyl reductase 2, kidney dicarbonyl reductase, short chain dehydrogenase/reductase family 20C, member 1
Protein Accession Number:
NP_057370.1
Protein Gi Number:
7705925
Ncbi Gene Id Number:
51181
Background Reference 1:
Nakagawa J, Ishikura S, Asami J, Isaji T, Usami N, Hara A, Sakurai T, Tsuritani K, Oda K, Takahashi M, Yoshimoto M, Otsuka N, Kitamura K. Molecular characterization of mammalian dicarbonyl/L-xylulose reductase and its localization in kidney J Biol Chem. 2002 May 17;277(20):17883-91

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-479

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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