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Western blot analysis of extracts of various cell lines, using DICER1 antibody (19-979) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DICER1 antibody (19-979) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using DICER1 Polyclonal Antibody (19-979) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using DICER1 antibody (19-979) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

DICER1 Antibody

CATALOG NUMBER: 19-979

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
IF: 1:100 - 1:200
Positive Control 1:
NCI-H460
Positive Control 2:
U-251MG
Positive Control 3:
HepG2
Positive Control 4:
MCF7
Predicted Molecular Weight:
Observed: 250kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DICER1
Additional Names:
DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, endoribonuclease Dicer, Dicer1, Dcr-1 homolog, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, helicase MOI, helicase with RNAse motif
Ncbi Gene Id Number:
23405
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-979

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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