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Western blot analysis of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody at 1:1000 at each lane.
Western blot analysis of DIO2 Antibody in MCF-7 cell line lysates (35ug/lane)
DIO2 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody at 1:1000 at each lane.

DIO2 Antibody

CATALOG NUMBER: 63-610

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This DIO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-191 amino acids from the Central region of human DIO2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DIO2
Additional Names:
Type II iodothyronine deiodinase, 5DII, DIOII, Type 2 DI, Type-II 5'-deiodinase, DIO2, ITDI2, TXDI2
Protein Accession Number:
Q92813
Protein Gi Number:
172045839
Ncbi Gene Id Number:
1734
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DIO2 belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3).
Background Reference 1:
He,B.,et.al., Prog. Neuropsychopharmacol. Biol. Psychiatry 33 (6), 986-990 (2009)
Background Reference 2:
Heemstra,K.A., et.al., J. Clin. Endocrinol. Metab. 94 (6), 2144-2150 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-610

List Size:
400 ul

List Price:

$495.00

Datasheet
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