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Western blot analysis of DIO3 Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of DIO3 Antibody in HepG2 cell line lysates (35ug/lane)

DIO3 Antibody

CATALOG NUMBER: 63-991

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DIO3
Additional Names:
Type III iodothyronine deiodinase, 5DIII, DIOIII, Type 3 DI, Type-III 5'-deiodinase, DIO3, ITDI3, TXDI3
Protein Accession Number:
P55073
Protein Gi Number:
172045679
Ncbi Gene Id Number:
1735
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Background Reference 1:
Bessho, K., et al. Eur. J. Pediatr. 169(2):215-221(2010)
Background Reference 2:
Wallace, C., et al. Nat. Genet. 42(1):68-71(2010)
Background Reference 3:
Aerts, G., et al. Endocrinology 150(11):5171-5180(2009)
Background Reference 4:
Panicker, V., et al. J. Clin. Endocrinol. Metab. 94(5):1623-1629(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-991

List Size:
400 ul

List Price:

$495.00

Datasheet
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