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Western blot analysis of DIPA Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with DIPA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DIPA Antibody in HepG2 cell line lysates (35ug/lane)

DIPA Antibody

CATALOG NUMBER: 63-788

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DIPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-96 amino acids from the Central region of human DIPA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCDC85B
Additional Names:
Coiled-coil domain-containing protein 85B, Hepatitis delta antigen-interacting protein A, Delta-interacting protein A, CCDC85B, DIPA
Protein Accession Number:
Q15834
Protein Gi Number:
92090801
Ncbi Gene Id Number:
11007
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DIPA functions as a transcriptional repressor. It may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. It may function in adipocyte differentiation, negatively regulating mitotic clonal expansion.
Background Reference 1:
Bezy,O., et.al., J. Biol. Chem. 280 (12), 11432-11438 (2005)
Background Reference 2:
Du,X.,et.al., Exp. Mol. Pathol. 81 (3), 184-190 (2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-788

List Size:
400 ul

List Price:

$495.00

Datasheet
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