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Western blot analysis of DIRAS3 in human testis tissue lysate with DIRAS3 antibody at 1 μg/ml.
Western blot analysis of DIRAS3 in human testis tissue lysate with DIRAS3 antibody at 1 μg/ml.
Trial Size

DIRAS3 Antibody

CATALOG NUMBER: 8139

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3.
The immunogen is located within the last 50 amino acids of DIRAS3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
DIRAS3 antibody is human specific.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 25 kDa
Observed: 24 kDa
Purification:
DIRAS3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DIRAS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DIRAS3
Additional Names:
DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2
Protein Accession Number:
NP_004666
Protein Gi Number:
4757772
Ncbi Gene Id Number:
9077
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).
Background Reference 1:
Yu Y, Xu F, Peng H, et al. NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas. Proc. Natl. Acad. Sci. USA 1999; 96:214-9.
Background Reference 2:
Lu Z, Luo RZ, Lu Y, et al. The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells. J. Clin. Invest. 2008; 118:3917-29.
Background Reference 3:
Klingauf M, Beck M, Berge U, et al. The tumour suppressor DiRas3 interacts with C-RAF and downregulates MEK activity to restrict cell migration. Biol. Cell 2013; 105:91-107.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8139

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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