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Western blot analysis of DIS3L2 in 3T3 cell lysate with DIS3L2 antibody at 1 μg/ml.
Immunohistochemistry of DIS3L2 in rat brain tissue with DIS3L2 antibody at 5 μg/mL.
Western blot analysis of DIS3L2 in 3T3 cell lysate with DIS3L2 antibody at 1 μg/ml.
Trial Size

DIS3L2 Antibody

CATALOG NUMBER: 8347

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
DIS3L2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DIS3L2.
The immunogen is located within amino acids 720 - 770 of DIS3L2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DIS3L2 antibody can be used for detection of DIS3L2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Specificity:
DIS3L2 antibody is human, mouse and rat reactive. At least three isoforms of DIS3L2 are known to exist; this antibody will only detect the longest isoform. DIS3L2 is predicted to not cross-react with DIS3 or DIS3L.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight:
Predicted: 97 kDa
Observed: 94 kDa
Purification:
DIS3L2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DIS3L2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DIS3L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DIS3L2
Additional Names:
DIS3 like 3'-5' exoribonuclease 2, FAM6A, PRLMNS
Protein Accession Number:
NP_689596
Protein Gi Number:
134288890
Ncbi Gene Id Number:
129563
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The exosome is involved in a multitude of cellular RNA processing and degradation events (1). DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species (2). DIS3L2 is a paralog of DIS3, but unlike DIS3 is located in the cytoplasm (3). DIS3L2 defines a novel eukaryotic RNA degradation pathway and preferentially targets uridylated RNA (4). DIS3L2 mutations have also been associated with sporadic Wilms tumors (3).
Background Reference 1:
Chen CY, Gherzi R, Ong SE, et al. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell 2001; 107:451-64.
Background Reference 2:
Brouwer R, Allmang C, Raijmakers R, et al. Three novel components of the human exosome. J. Biol. Chem. 2001; 276:6177-84.
Background Reference 3:
Astuti D, Morris MR, Cooper WN, et al. Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility. Nat. Genet. 2012; 44:277-84.
Background Reference 4:
Malecki M, Viegas SC, Carneiro T, et al. The exonuclease Dis3L2 defines a novel eukaryotic RNA degradation pathway. EMBO J. 2013; 32:1842-54.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8347

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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