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Western blot analysis of DISP1 in 3T3 cell lysate with DISP1 antibody at 1 μg/mL.
Immunohistochemistry of DISP1 in human kidney tissue with DISP1 antibody at 2.5 μg/mL.
Immunofluorescence of DISP1 in human kidney tissue with DISP1 antibody at 20 μg/mL.
Western blot analysis of DISP1 in 3T3 cell lysate with DISP1 antibody at 1 μg/mL.
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DISP1 Antibody

CATALOG NUMBER: 6629

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
DISP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human DISP1.
The immunogen is located within amino acids 20 - 70 of DISP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DISP1 antibody can be used for detection of DISP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of DISP1 are known to exist; this antibody will detect both isoforms. DISP1 antibody is predicted to not cross-react with EMX1.
Positive Control 1:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
DISP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
DISP1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DISP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DISP1
Additional Names:
DISP1 Antibody: DISPA, DISPA, Protein dispatched homolog 1
Protein Accession Number:
NP_116279
Protein Gi Number:
25952134
Ncbi Gene Id Number:
84976
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DISP1 Antibody: DISP1 is the mammalian homolog of the Drosophila Dispatched segment-polarity gene and a key regulator of the Hedgehog (Hh) signaling pathway during embryonic development. DISP1 is required to move Shh from site of synthesis during embryogenesis; mutations in the gene result in lethality at mid-gestation and prevents specification of ventral cell types in the neural tube. Recent results have shown that DISP1 mediates the basolateral secretion of Shh and regulates growth of mammalian long bones through the control of Ihh.
Background Reference 1:
Caspary T, Garcia-Garcia MJ, Huangfu D, et al. Mouse dispatched homolog1 is required for long-range, but not juxtacrine, Hh signaling. Curr. Biol. 2002; 12:1628-32
Background Reference 2:
Etheridge LA, Crawford TQ, Zhang S, et al. Evidence for a role of vertebrate Disp1 in long-range Shh signaling. Development 2010; 137:133-40.
Background Reference 3:
Tsiairis CD and McMahon AP. Disp1 regulates growth of mammalian long bones through the control of Ihh distribution. Dev. Biol. 2008; 317:480-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6629

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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