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45-482 (2.5ug/ml) staining of U87. Detected by immunofluorescence. Data provided by Dr. Aashir Awan, University of Madrid, Spain.
45-482 (2.5ug/ml) staining of U87. Detected by immunofluorescence. Data provided by Dr. Aashir Awan, University of Madrid, Spain.

Dispatched homolog 1 Antibody

CATALOG NUMBER: 45-482

Clonality:
Polyclonal
Tested Applications:
ELISA, IF
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: C-SELSGESLLIKTL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1ug/ml.Immunofluorescence: Cell membrane staining in cell line U87. These data corresponds to earlier findings in literature with different antibodies (Etheridge et al, Development. 2010 Jan;137(1):133-40; PMID: 20023168).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DISP1
Additional Names:
DISP1, DISPA, MGC13130, MGC16796, DKFZP434I0428, dispatched A, DISP1, dispatched homolog 1, dispatched homolog 1 (Drosophila), FLJ43740, MGC104180
Protein Accession Number:
NP_116279.2
Protein Gi Number:
25952134
Ncbi Gene Id Number:
84976
Background Reference 1:
Burke R, Nellen D, Bellotto M, Hafen E, Senti KA, Dickson BJ, Basler K. Dispatched, a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells. Cell. 1999 Dec 23;99(7):803-15.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-482

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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