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42-274 (0.5ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-274 (0.5ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DIXDC1 Antibody

CATALOG NUMBER: 42-274

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DVSYHQVDLERE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 53.9kDa according to NP_219493.1). Recommended concentration: 0.5-2ug/ml.
Specificity:
This antibody is expected to recognize both isoforms (NP_001033043.1; NP_219493.1).
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DIXDC1
Additional Names:
DIXDC1, DIX domain containing 1, CCD1, KIAA1735
Protein Accession Number:
NP_001033043.1,NP_219493.1
Protein Gi Number:
83816971
Ncbi Gene Id Number:
85458
Background Reference 1:
Wu Y, Jing X, Ma X, Wu Y, Ding X, Fan W, Fan M. DIXDC1 co-localizes and interacts with gamma-tubulin in HEK293 cells. Cell biology international 2009 Jun 33 (6): 697-701.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-274

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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