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Western blot analysis of extracts of various cell lines, using DKC1 antibody (18-354) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using DKC1 Antibody (18-354) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human cancer using DKC1 Antibody (18-354) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DKC1 Antibody (18-354) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using DKC1 antibody (18-354). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using DKC1 antibody (18-354) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

DKC1 Antibody

CATALOG NUMBER: 18-354

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 245-514 of human DKC1 (NP_001354.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
U-251MG
Positive Control 2:
Mouse testis
Predicted Molecular Weight:
Observed: 43kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DKC1
Additional Names:
DKC1, dyskeratosis congenita 1, dyskerin, DKC, NAP57, NOLA4, XAP101
Ncbi Gene Id Number:
1736
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-354

List Size:
100 uL

List Price:

$436.00

Datasheet
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