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Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug human PLC/PRF5 cell lysate, Lane 2: 30ug DKK1 PLC/PRF5 cell lysate ).
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug human PLC/PRF5 cell lysate, Lane 2: 30ug DKK1 PLC/PRF5 cell lysate ).

DKK1 Antibody

CATALOG NUMBER: 30-346

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DKK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DKK1 antibody can be used for detection of DKK1 by ELISA at 1:312500. DKK1 antibody can be used for detection of DKK1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
26 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DKK1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DKK1
Additional Names:
DKK1, DKK-1, SK
Protein Accession Number:
NP_036374
Protein Gi Number:
7110719
Ncbi Gene Id Number:
22943
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DKK1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Background Reference 1:
Ai, M., (2005) Mol. Cell. Biol. 25 (12), 4946-4955.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-346

List Size:
100 ul

List Price:

$473.00

Datasheet
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