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45-485 staining (2ug/ml) of Human Cerebellum lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-485 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.<strong>This data is from a previous batch, not on sale.</strong>
45-485 staining (2ug/ml) of Human Cerebellum lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

DKK3 Antibody

CATALOG NUMBER: 45-485

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-AAAALLGGEEI
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of38.4xkDa according to NP_037385.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Specificity:
NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein.
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DKK3
Additional Names:
DKK3, REIC, dickkopf homolog 3 (Xenopus laevis), DKK-3, RIG-like 5-6, RIG-like 7-1, dickkopf 3, Dickkopf gene 3, dickkopf (Xenopus laevis) homolog 3, dickkopf homolog 3, RIG, regulated in glioma
Protein Accession Number:
NP_037385.2,NP_056965.3,NP_001018067.1
Protein Gi Number:
40548393
Ncbi Gene Id Number:
27122
Background Reference 1:
Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA. Functional and structural diversity of the human Dickkopf gene family. Gene. 1999 Oct 1;238(2):301-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-485

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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