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Antibody is used in Western blot to detect DKKL1 in mouse testis tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect DKKL1 in mouse testis tissue lysate.

DKKL1 Antibody

CATALOG NUMBER: 58-171

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DKKL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-242 amino acids from the C-terminal region of human DKKL1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
27 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DKKL1
Additional Names:
Dickkopf-like protein 1, Cancer/testis antigen 34, CT34, Protein soggy-1, SGY-1, DKKL1, SGY1
Protein Accession Number:
Q9UK85
Protein Gi Number:
13124528
Ncbi Gene Id Number:
27120
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The dickkopf protein family interacts with the Wnt signaling pathway and its members are characterized by two conserved cysteine-rich domains. This gene encodes a secreted protein that has high similarity to the N-terminus of the dickkopf-3 protein and moderate similarity to that protein's C-terminus though the C-terminal cysteine residues are not conserved.
Background Reference 1:
Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Krupnik, V.E., et al., Gene 238(2):301-313 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-171

List Size:
400 ul

List Price:

$495.00

Datasheet
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