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Antibody used in WB on Human Jurkat 5.0 ug/ml.
Antibody used in WB on Human Huh7, HepG2 at 1:200.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat 5.0 ug/ml.

DLAT Antibody

CATALOG NUMBER: 29-681

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DLAT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
DLAT antibody can be used for detection of DLAT by ELISA at 1:312500. DLAT antibody can be used for detection of DLAT by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
71 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DLAT antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DLAT
Additional Names:
DLAT, DLTA, PDC-E2, PDCE2
Protein Accession Number:
NP_001922
Protein Gi Number:
31711992
Ncbi Gene Id Number:
1737
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DLAT is dihydrolipoamide acetyltransferase, the E2 subunit of the mammalian pyruvate dehydrogenase complex of the inner mitochondrial membrane. Patients with primary biliary cirrhosis show autoantibodies to DLAT.The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.
Background Reference 1:
Head, R.A., (2005) Ann. Neurol. 58 (2), 234-241.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-681

List Size:
100 ul

List Price:

$473.00

Datasheet
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