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45-487 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle staining. The nuclear stain is
45-487 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai
45-487 (4ug/ml) staining of paraffin embedded Mouse Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
45-487 Negative Control showing staining of paraffin embedded Mouse Adrenal Gland, with no primary antibody.
45-487 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle staining. The nuclear stain is

DLC1 Antibody

CATALOG NUMBER: 45-487

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Pig
Immunogen:
The immunogen for this antibody is: SVAIRKRSWEEHC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:Paraffin embedded Mouse Adrenal Gland. Recommended concentration: 4ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U251 and U2OS cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2).
Positive Control 1:
Cat. No. XBL-11050 - Human Adrenal Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DLC1
Additional Names:
DLC1, HP, ARHGAP7, STARD12, FLJ21120, p122-RhoGAP, deleted in liver cancer 1, Rho-GTPase-activating protein 7, START domain containing protein 12, StAR-related lipid, transfer protein 12, StAR-related lipid transfer (START) domain containing 12, deleted in liver cancer 1 variant 2
Protein Accession Number:
NP_872584.2,NP_079043.3
Protein Gi Number:
33188433
Ncbi Gene Id Number:
10395
Background Reference 1:
Wilson PJ, McGlinn E, Marsh A, Evans T, Arnold J, Wright K, Biden K, Young J, Wainwright B, Wicking C, Chenevix-Trench G. Sequence variants of DLC1 in colorectal and ovarian tumours. Hum Mutat. 2000;15(2):156-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-487

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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