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Western blot analysis of extracts of various cell lines, using DLG1 antibody (23-649) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using DLG1 antibody (23-649) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DLG1 antibody (23-649) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DLG1 antibody (23-649) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using DLG1 antibody (23-649) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

DLG1 Antibody

CATALOG NUMBER: 23-649

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
U-87MG
Positive Control 2:
LO2
Positive Control 3:
HeLa
Positive Control 4:
Jurkat
Predicted Molecular Weight:
Observed: 114kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DLG1
Additional Names:
DLG1, discs, large homolog 1 (Drosophila), DKFZp761P0818, DKFZp781B0426, DLGH1, SAP97, dJ1061C18.1.1, hdlg, discs large homolog 1, large homolog 1, presynaptic protein SAP97, synapse-associated protein 97, SAP-97
Ncbi Gene Id Number:
1739
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-649

List Size:
100 uL

List Price:

$436.00

Datasheet
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