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46-831 (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-831 (0.3ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DLG1 Antibody

CATALOG NUMBER: 46-831

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat,
Immunogen:
The immunogen for this antibody is: C-NSKTRDKGEIPDD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 150kDa band observed in lysates of cell line KELLY (calculated MW of 103kDa according to NP_004078.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001091894.1and NP_004078.2).
Positive Control 1:
KELLY (Human neuroblastoma cell line) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DLG1
Additional Names:
DLG1 , discs, large homolog 1 (Drosophila) , DKFZp761P0818 , DKFZp781B0426 , DLGH1 , SAP97 , dJ1061C18.1.1 , hdlg, discs large homolog 1 , discs, large homolog 1 , presynaptic protein SAP97 , synapse-associated protein 97
Protein Accession Number:
NP_001091894.1,NP_004078.2
Protein Gi Number:
148539628
Ncbi Gene Id Number:
1739
Background Reference 1:
Mahoney ZX, Sammut B, Xavier RJ, Cunningham J, Go G, Brim KL, Stappenbeck TS, Miner JH, Swat W. Discs-large homolog 1 regulates smooth muscle orientation in the mouse ureter. Proc Natl Acad Sci USA. 2006 Dec 26;103(52):19872-7. Epub 2006 Dec 15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-831

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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