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Western blot analysis of DLG7 Antibody in 293 cell line lysates (35ug/lane)
DLG7 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DLG7 Antibody in 293 cell line lysates (35ug/lane)

DLG7 Antibody

CATALOG NUMBER: 61-521

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DLG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-258 amino acids from the Central region of human DLG7.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
95 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DLGAP5
Additional Names:
Disks large-associated protein 5, DAP-5, Discs large homolog 7, Disks large-associated protein DLG7, Hepatoma up-regulated protein, HURP, DLGAP5, DLG7, KIAA0008
Protein Accession Number:
Q15398
Protein Gi Number:
82592583
Ncbi Gene Id Number:
9787
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DLG7 is potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. DLG7 is mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. DLG7 is key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Background Reference 1:
de Reynies, A., et al. J. Clin. Oncol. 27(7):1108-1115(2009) Wong, J., et al. Mol. Biol. Cell 19(5):2083-2091(2008) Gudmundsson, K.O., et al. Stem Cells 25(6):1498-1506(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-521

List Size:
400 ul

List Price:

$495.00

Datasheet
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