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Western blot analysis of DLK2 Antibody in HL-60 cell line lysates (35ug/lane)
DLK2 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DLK2 using rabbit polyclonal DLK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DLK2 gene.
Western blot analysis of DLK2 Antibody in HL-60 cell line lysates (35ug/lane)

DLK2 Antibody

CATALOG NUMBER: 63-748

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of human DLK2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DLK2
Additional Names:
Protein delta homolog 2, DLK-2, Epidermal growth factor-like protein 9, EGF-like protein 9, DLK2, EGFL9
Protein Accession Number:
Q6UY11
Protein Gi Number:
55976806
Ncbi Gene Id Number:
65989
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DLK2 (Delta like protein 2) affects adipogenesis of 3T3-L1 preadipocytes and mesenchymal C3H10T1/2 cells suggesting a role as a regulator of adipogenesis.
Background Reference 1:
Mural R.J., et.al., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-748

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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