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46-984 (2ug/ml) staining of Rat Pancreas lysate (A) + Peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-984 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. Actin filaments wer
46-984 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-984 (2ug/ml) staining of Rat Pancreas lysate (A) + Peptide (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.

DLL1 Antibody

CATALOG NUMBER: 46-984

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-ATQRHLTVGEEWSQD
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5kDa according to Mouse NP_031891.2 and 77.4kDa according to Rat NP_114452.1). An additional band of nknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration:0.3-7ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Positive Control 1:
Cat. No. 1461 - Rat Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DLL1
Additional Names:
DLL1, delta-like 1 (Drosophila), DELTA1, DL1, Delta, OTTHUMP00000017690, delta (Drosophila)-like 1, delta-like 1, delta-like 1 protein
Protein Accession Number:
NP_005609.3
Protein Gi Number:
110735443
Ncbi Gene Id Number:
28514
Background Reference 1:
Ivey KN, Muth A, Arnold J, King FW, Yeh RF, Fish JE, Hsiao EC, Schwartz RJ, Conklin BR, Bernstein HS, Srivastava D. MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. 2008 Mar 2 (3): 219-29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-984

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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