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Western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
Western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).

DMRTA2 Antibody

CATALOG NUMBER: 60-806

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Zebrafish
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DMRTA2
Additional Names:
Doublesex- and mab-3-related transcription factor A2, Doublesex- and mab-3-related transcription factor 5, DMRTA2, DMRT5
Protein Accession Number:
Q96SC8
Protein Gi Number:
189044724
Ncbi Gene Id Number:
63950
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DMRTA2 may be involved in sexual development.
Background Reference 1:
Ottolenghi, C., et al. Genomics 79(3):333-343(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-806

List Size:
400 ul

List Price:

$495.00

Datasheet
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