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Western blot analysis of DNAJA4 in human colon tissue lysate with DNAJA4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of DNAJA4 in human colon tissue lysate with DNAJA4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

DNAJA4 Antibody

CATALOG NUMBER: 8141

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (87%)
Immunogen:
DNAJA4 antibody was raised against a 15 amino acid peptide near the center of human DNAJA4.
The immunogen is located within amino acids 210 - 260 of DNAJA4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DNAJA4 antibody can be used for detection of DNAJA4 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
DNAJA4 antibody is human, mouse, and rat reactive. At least three isoforms of DNAJA4 are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 41, 44, 47 kDa
Observed: 40 kDa
Purification:
DNAJA4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DNAJA4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DNAJA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DNAJA4
Additional Names:
DnaJ homolog subfamily A member 4, MST104, MSTP104, PRO1472
Protein Accession Number:
NP_061072
Protein Gi Number:
194328758
Ncbi Gene Id Number:
55466
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The DNAJA4 protein is the mammalian homolog of the chaperone HSP40 (1) and acts as a co-chaperone with the heat shock protein HSP70 (2). DNAJA4 is highly expressed in heart and testis, accounting for approximately 1% of total protein in mouse heart (2). DNAJA4 is also regulated by the sterol regulatory element binding transcription factor 2 (SREBF2) and plays a role in the synthesis of cholesterol (3).
Background Reference 1:
Ohtsuka K and Hata M. Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature. Cell Stress Chaperones 2000; 5:98-112.
Background Reference 2:
Abdul KM, Terada K, Gotoh T, et al. Characterization and functional analysis of a heart-enriched DnaJ / Hsp40 homolog dj4/DjA4. Cell Stress Chaperones 2002; 7:156-66.
Background Reference 3:
Robichon C, Varret M, Le Liepvre X, et al. DnajA4 is a SREBP-regulated chaperone involved in the cholesterol biosynthesis pathway. Biochim. Biophys. Acta 2006; 1761:1107-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8141

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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