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Western blot analysis of DNAJB6 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate.
DNAJB6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of DNAJB6 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in Hela cell line lysate.

DNAJB6 Antibody

CATALOG NUMBER: 56-462

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DNAJB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-247 amino acids from the Central region of human DNAJB6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DNAJB6
Additional Names:
DnaJ homolog subfamily B member 6, HHDJ1, Heat shock protein J2, HSJ-2, MRJ, MSJ-1, DNAJB6, HSJ2, MRJ, MSJ1
Protein Accession Number:
O75190
Ncbi Gene Id Number:
10049
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the DNAJ protein family.DNAJ family members are characterized by a highly conserved aminoacid stretch called the 'J-domain' and function as one of the twomajor classes of molecular chaperones involved in a wide range ofcellular events, such as protein folding and oligomeric proteincomplex assembly. This family member may also play a role inpolyglutamine aggregation in specific neurons. Alternative splicingof this gene results in multiple transcript variants; however, notall variants have been fully described.
Background Reference 1:
Mitra, A., et al. J. Biol. Chem. 285(32):24686-24694(2010)
Background Reference 2:
Edo De Bock, C., et al. Int. J. Oncol. 36(5):1155-1163(2010)
Background Reference 3:
Hageman, J., et al. Mol. Cell 37(3):355-369(2010)
Background Reference 4:
Lowe, J.K., et al. PLoS Genet.(2), E1000365 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-462

List Size:
400 ul

List Price:

$495.00

Datasheet
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