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<strong> WB Validation of DNAJC13 with Mouse Samples </strong><br>Loading: 15 μg of lysate per lane.Detection Antibodies: DNAJC13, 8109, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution, incubated at RT for 1 hr.
Immunohistochemistry of DNAJC13 in human heart tissue with DNAJC13 antibody at 5 μg/mL.
WB Validation of DNAJC13 with Mouse Samples
Loading: 15 μg of lysate per lane.Detection Antibodies: DNAJC13, 8109, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution, incubated at RT for 1 hr.
Trial Size

DNAJC13 Antibody

CATALOG NUMBER: 8109

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13.
The immunogen is located within amino acids 1940 - 1990 of DNAJC13.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.
Positive Control 1:
Cat. No. 1411 - Mouse Colon Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Positive Control 3:
Cat. No. 1212 - 3T3 Cell Lysate
Predicted Molecular Weight:
Predicted: 249 kDa
Observed: 249 kDa
Purification:
DNAJC13 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DNAJC13 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DNAJC13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DNAJC13
Additional Names:
DNAJ homolog subfamily C member 13, receptor-mediated endocytosis 8, RME8
Protein Accession Number:
NP_056083
Protein Gi Number:
112421122
Ncbi Gene Id Number:
23317
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).
Background Reference 1:
Girard M, Poupon V, Blondeau F, et al. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J. Biol. Chem. 2005; 280:40135-43.
Background Reference 2:
Fujibayashi A, Taguchi T, Misaki R, et al. Human RME-8 is involved in membrane trafficking through early endosomes. Cell Struct. Funct. 2008; 33:35-50.
Background Reference 3:
Girard M and McPherson PS. RME-8 regulates trafficking of the epidermal growth factor receptor. FEBS Lett. 2008; 582:961-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8109

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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