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Western blot analysis of extracts of various cell lines, using DNAJC19 antibody (19-537) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using DNAJC19 antibody (19-537) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DNAJC19 antibody (19-537) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Hela cells using DNAJC19 antibody (19-537) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using DNAJC19 antibody (19-537) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

DNAJC19 Antibody

CATALOG NUMBER: 19-537

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human DNAJC19 (NP_660304.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
B cells
Positive Control 3:
HeLa
Positive Control 4:
HT-1080
Positive Control 5:
HL-60
Positive Control 6:
Mouse pancreas
Predicted Molecular Weight:
Observed: 12kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DNAJC19
Additional Names:
Mitochondrial import inner membrane translocase subunit TIM14, DnaJ homolog subfamily C member 19, DNAJC19, TIM14, TIMM14
Ncbi Gene Id Number:
131118
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is thought to be part of a complex involved in the ATP-dependent transport of transit peptide-containing proteins from the inner cell membrane to the mitochondrial matrix. Defects in this gene are a cause of 3-methylglutaconic aciduria type 5 (MGA5), also known as dilated cardiomyopathy with ataxia (DCMA). Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 2, 6, 10, 14 and 19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-537

List Size:
100 uL

List Price:

$436.00

Datasheet
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