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43-177 (0.5ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-177 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-177 (0.5ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Dnajc5 Antibody

CATALOG NUMBER: 43-177

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SATETTQLTADSHPS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 20kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.1kDa according to NP_058055.1). Recommended concentration: 0.5-1.5ug/ml. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 20kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Dnajc5
Additional Names:
Dnajc5, DnaJ (Hsp40) homolog, subfamily C, member 5, 2610314I24Rik, AU018536, Csp, cysteine string protein, dnaJ homolog subfamily C member 5
Protein Accession Number:
NP_058055.1
Protein Gi Number:
7949027
Ncbi Gene Id Number:
80331
Background Reference 1:
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC.CSPα knockout causes neurodegeneration by impairing SNAP-25 function.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-177

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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