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Western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).
DNAJC6 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).

DNAJC6 Antibody

CATALOG NUMBER: 64-222

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This DNAJC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-281 amino acids from the Central region of human DNAJC6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
100 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DNAJC6
Additional Names:
Putative tyrosine-protein phosphatase auxilin, DnaJ homolog subfamily C member 6, DNAJC6, KIAA0473
Protein Accession Number:
O75061
Ncbi Gene Id Number:
9829
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain
Background Reference 1:
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Background Reference 2:
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Background Reference 3:
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Background Reference 4:
Hirst, J., et al. Traffic 9(8):1354-1371(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-222

List Size:
400 ul

List Price:

$495.00

Datasheet
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