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Western blot analysis of extracts of various cell lines, using DNAJC7 antibody (18-809) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using DNAJC7 antibody (18-809) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

DNAJC7 Antibody

CATALOG NUMBER: 18-809

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-380 of human DNAJC7 (NP_003306.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:1000 - 1:2000
Positive Control 1:
A-431
Positive Control 2:
293T
Positive Control 3:
K-562
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DNAJC7
Additional Names:
DJ11, DJC7, TPR2, TTC2, dnaJ homolog subfamily C member 7, DnaJ (Hsp40) homolog, subfamily C, member 7, TPR repeat protein 2, tetratricopeptide repeat domain 2, tetratricopeptide repeat protein 2
Ncbi Gene Id Number:
7266
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the DNAJ heat shock protein 40 family of proteins that is characterized by two N-terminal tetratricopeptide repeat domains and a C-terminal DNAJ domain. This protein binds the chaperone proteins heat shock proteins 70 and 90 in an ATP-dependent manner and may function as a co-chaperone. Pseudogenes of this gene are found on chromosomes 1 and 6. Alternate splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-809

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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