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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

DNAJC9 Antibody

CATALOG NUMBER: 56-746

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DNAJC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human DNAJC9.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DNAJC9
Additional Names:
DnaJ homolog subfamily C member 9, DnaJ protein SB73, DNAJC9
Protein Accession Number:
Q8WXX5
Protein Gi Number:
52782787
Ncbi Gene Id Number:
23234
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNAJC9 is upregulated at both mRNA and protein levels after various mitogenic and stress stimulations. DNAJC9 is mainly localized in cell nuclei under normal culture conditions, and transported into cytoplasm and plasma membrane upon heat shock stress through a non-classical and lipid-dependent pathway. DNAJC9 can interact with HSP70s and activate the ATPase activity of HSP70s, both of which are dependent on the J domain. Research has indicated that DNAJC9 is a novel cochaperone for HSP70s.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Pan, Z., et al. Neoplasma 57(2):123-128(2010)
Background Reference 3:
Han, C., et al. Biochem. Biophys. Res. Commun. 353(2):280-285(2007)
Background Reference 4:
Takamura, Y., et al. Biochem. Biophys. Res. Commun. 285(2):387-392(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-746

List Size:
400 ul

List Price:

$495.00

Datasheet
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