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Western Blot at 1:2000 dilution + T47D whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:2000 dilution + T47D whole cell lysates Lysates/proteins at 20 ug per lane.

Dnmt3b Antibody

CATALOG NUMBER: 55-145

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
96 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DNMT3B
Additional Names:
DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, MHsaIIIB, DNMT3B
Protein Accession Number:
Q9UBC3
Protein Gi Number:
17375667
Ncbi Gene Id Number:
1789
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.
Background Reference 1:
Okano, M., et al., Cell 99(3):247-257 (1999). Yin, B., et al., Zhongguo Yi Xue Ke Xue Yuan Xue Bao 21(6):431-438 (1999). Okano, M., et al., Nat. Genet. 19(3):219-220 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-145

List Size:
400 ul

List Price:

$495.00

Datasheet
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