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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).
DOC2A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

DOC2A Antibody

CATALOG NUMBER: 56-685

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This DOC2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human DOC2A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DOC2A
Additional Names:
Double C2-like domain-containing protein alpha, Doc2, Doc2-alpha, DOC2A
Protein Accession Number:
Q14183
Protein Gi Number:
150421541
Ncbi Gene Id Number:
8448
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
There are at least two protein isoforms of the Double C2protein, namely alpha (DOC2A) and beta (DOC2B), which contain twoC2-like domains. DOC2A and DOC2B are encoded by different genes;these genes are at times confused with the unrelated DAB2 genewhich was initially named DOC-2. DOC2A is mainly expressed in brainand is suggested to be involved in Ca(2+)-dependentneurotransmitter release.
Background Reference 1:
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Background Reference 2:
Glessner, J.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(23):10584-10589(2010)
Background Reference 3:
Guilmatre, A., et al. Arch. Gen. Psychiatry 66(9):947-956(2009)
Background Reference 4:
Kumar, R.A., et al. PLoS ONE(2), E4582 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-685

List Size:
400 ul

List Price:

$495.00

Datasheet
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