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45-489 staining (4ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
45-489 staining (4ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

DOCK1 Antibody

CATALOG NUMBER: 45-489

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TTRKQTSVDSGIVQ
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot:Approx 150-200kDa band seen in A431 cell lysate. Recommended for use at 3-4ug/ml.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DOCK1
Additional Names:
DOCK1, DOCK180, dedicator of cyto-kinesis 1, RP11-82L9.1, ced5
Protein Accession Number:
NP_001371.1
Protein Gi Number:
4503355
Ncbi Gene Id Number:
1793
Background Reference 1:
Hasegawa H, Kiyokawa E, Tanaka S, Nagashima K, Gotoh N, Shibuya M, Kurata T, Matsuda M. DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane. Mol Cell Biol 1996 Apr;16(4):1770-6

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-489

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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