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Western blot analysis of DOCK8 in EL4 cell lysate with DOCK8 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of DOCK8 in human spleen tissue with DOCK8 antibody at 5 μg/mL.
Western blot analysis of DOCK8 in EL4 cell lysate with DOCK8 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

DOCK8 Antibody

CATALOG NUMBER: 7847

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
DOCK8 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DOCK8.
The immunogen is located within the last 50 amino acids of DOCK8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 166, 220, 231 kDa
Observed: 220, 230 kDa
Purification:
DOCK8 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DOCK8 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DOCK8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DOCK8
Additional Names:
DOCK8 Antibody: MRD2, ZIR8, HEL-205, Dedicator of cytokinesis protein 8
Protein Accession Number:
NP_982272
Protein Gi Number:
238231392
Ncbi Gene Id Number:
81704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).
Background Reference 1:
Ruusala A and Aspenstrom P. Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology. FEBS Lett. 2004; 572:159-66.
Background Reference 2:
Randall KL, Lambe T, Goodnow CC, et al. The essential role of DOCK8 in humoral immunity. Dis. Markers 2010; 29:141-50.
Background Reference 3:
Engelhardt KR, McGhee S, Sinkler S, et al. Large deletions and point mutations involving the dedicator of cytokinesis 8 (DOCK8) in the autosomal recessive form of hyper-IgE syndrome. J. Allergy Clin. Immunol. 2009; 124:1289-302.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7847

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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