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IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG 1 antibody (DG1/1484). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free DOG 1 Antibody (clone DG1/1484). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG 1 antibody (DG1/1484). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

DOG1 Antibody [DG1/1484]

CATALOG NUMBER: 33-698

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A recombinant human protein corresponding to amino acids 2-101 of human DOG1/ANO1 was used as the immunogen for this DOG 1 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG 1 antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
ANO1
Additional Names:
TMEM16A Antibody: DOG1, TAOS2, ORAOV2, TMEM16A, DOG1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1
Ncbi Gene Id Number:
55107
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
DOG 1 is expressed in the human gastrointestinal epithelium and is highly expressed in the gastrointestinal interstitial cells of Cajal, where it plays an important role in epithelial chloride secretion mediating intestinal motility. [Wiki]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-698

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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