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HEK293 overexpressing DOK5 and probed with 45-491 (mock transfection in first lane).
45-491 (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
HEK293 overexpressing DOK5 and probed with 45-491 (mock transfection in first lane).

DOK5 Antibody

CATALOG NUMBER: 45-491

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CLHRTETFPAYRSEH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing DOK5 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5kDa according to NP_060901.2. Recommended concentration, 1-3ug/ml.Immunohistochemistry:In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells. Recommended concentration, 1-2ug/ml.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 1315 - Human Tonsil Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DOK5
Additional Names:
DOK5, MGC16926, C20orf180, docking protein 5, downstream of tyrosine kinase 5, chromosome 20 open reading frame 180
Protein Accession Number:
NP_060901.2
Protein Gi Number:
29544726
Ncbi Gene Id Number:
55816
Background Reference 1:
Cai D, Dhe-Paganon S, Melendez PA, Lee J, Shoelson SE. Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5. J Biol Chem. 2003 Jul 11;278(28):25323-30. Epub 2003 May 01.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-491

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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