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Formalin-fixed, paraffin-embedded human tonsil stained with Double Stranded DNA antibody (SPM603)
Flow cytometry testing of fixed and permeabilized human Jurkat cells with dsDNA antibody (clone SPM603); Red=isotype control, Blue= dsDNA antibody.
Formalin-fixed, paraffin-embedded human tonsil stained with Double Stranded DNA antibody (SPM603)

Double Stranded DNA Antibody [SPM603]

CATALOG NUMBER: 33-534

Clonality:
Monoclonal
Tested Applications:
Flow, ICC, IF, IHC-P
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, ICC, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RT
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG3, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-534

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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