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Western blot analysis of extract from Molt-4 and HeLa cells using Doublecortin Antibody.
Western blot analysis of extract from Molt-4 and HeLa cells using Doublecortin Antibody.

Doublecortin Antibody

CATALOG NUMBER: 79-731

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Doublecortin antibody was raised against a peptide sequence around aa.413~417 ( P-T-S-P-G) derived from Human Doublecortin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total Doublecortin protein.
Predicted Molecular Weight:
40 - 45 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
DCX
Additional Names:
DC, DBCN, LISX, SCLH, XLIS, DCX, DC
Protein Accession Number:
NP_000546.2
Protein Gi Number:
30181246
Ncbi Gene Id Number:
1641
Background:
Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
Background Reference 1:
Des Portes V., Pinard J. -M., Billuart P., Vinet M. -C., Koulakoff A., Carrie A., Gelot A., Dupuis E., Motte J., Berwald-Netter Y., Catala M., Kahn A., Beldjord C., Chelly J. Cell 92:51-61 (1998) Pilz D. T., Matsumoto N., Minnerath S. R., Mills P., Gleeson J. G., Allen K. M., Walsh C. A., Barkovich A. J., Dobyns W. B., Ledbetter D. H., Ross M. E. Hum. Mol. Genet. 7:2029-2037 (1998)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-731

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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