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Western blot analysis of DPAGT1 in mouse kidney tissue lysate with DPAGT1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of DPAGT1 in human kidney tissue with DPAGT1 antibody at 2.5 μg/mL.
Immunofluorescence of DPAGT1 in human kidney tissue with DPAGT1 antibody at 20 μg/mL.
Western blot analysis of DPAGT1 in mouse kidney tissue lysate with DPAGT1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

DPAGT1 Antibody

CATALOG NUMBER: 6485

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1.
The immunogen is located within amino acids 30 - 80 of DPAGT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of DPAGT1 are known to exist; this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
DPAGT1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DPAGT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DPAGT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DPAGT1
Additional Names:
DPAGT1 Antibody: GPT, ALG7, DGPT, G1PT, UAGT, UGAT, CDG1J, DPAGT, CDG-Ij, CMSTA2, DPAGT2, D11S366, UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, GlcNAc-1-P transferase
Protein Accession Number:
NP_001373
Protein Gi Number:
42794009
Ncbi Gene Id Number:
1798
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DPAGT1 Antibody: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.
Background Reference 1:
Wu X, Rush JS, Karaoglu D, et al. Deficiency of UDP-GlcNAc:Dolichol Phosphate N-Acetylglucosamine-1 Phosphate Transferase (DPAGT1) causes a novel congenital disorder of glycosylation type Ij. Hum. Mutat. 2003; 22:144-50.
Background Reference 2:
Bretthauer RK. Structure, expression, and regulation of UDP-GlcNAc:dolichol phosphate GlcNAc-1-phosphate transferase (DPAGT1). Curr. Drug Targets 2009; 10:477-82
Background Reference 3:
Sengupta PK, Bouchie MP, and Kukuruzinska MA. N-glycosylation gene DPAGT1 is a target of the Wnt/beta-catenin signaling pathway. J. Biol. Chem. 2010; 285:31164-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6485

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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