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Western blot analysis of DPF3 in mouse brain tissue lysate with DPF3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of DPF3 in human brain tissue with DPF3 antibody at 5 μg/mL.
Immunofluorescence of DPF3 in human brain tissue with DPF3 antibody at 20 μg/mL.
Western blot analysis of DPF3 in mouse brain tissue lysate with DPF3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

DPF3 Antibody

CATALOG NUMBER: 7559

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (83%)
Immunogen:
DPF3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DPF3.
The immunogen is located within amino acids 220 - 270 of DPF3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 39 kDa
Observed: 50 kDa
Purification:
DPF3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DPF3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DPF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DPF3
Additional Names:
DPF3 Antibody: CERD4, BAF45C, CERD4, Zinc finger protein DPF3, BRG1-associated factor 45C
Protein Accession Number:
NP_036206
Protein Gi Number:
148762958
Ncbi Gene Id Number:
8110
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The DPF3 protein, also known as Cerd4, is a member of the d4 gene family of transcription modulators that also includes DPF1/Neud4 and DPF2/Requiem (1). DPF3 has been shown to be a epigenetic key factor for heart and muscle development and can bind to methylated and acetylated lysine residues of histone 3 and 4, suggesting that DPF3 may play a role in recruiting chromatin remodeling complexes to acetylated histones (2). Two isoforms of DPF3, DPF3a and DPF3b, are required as transcriptional co-activators in SWI/SNF complex-dependent activation of the NF-kappaB RelA/p50 heterodimer (3).
Background Reference 1:
Ninkina NN, Mertsalov IB, Kulikova DA, et al. Cerd4, third member of the d4 gene family: expression and organization of genomic locus. Mamm. Genome 2001; 12:862-6.
Background Reference 2:
Lange M, Kaynak B, Forster UB. Regulation of muscle development by DBF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex. Genes Dev. 2008; 22:2370-84.
Background Reference 3:
Ishizaka A, Mizutani T, Kobayashi K, et al. Double plant homeodomain (PHD) finger proteins DPF3a and -3b are required as transcriptional co-activators in SWI/SNF complex-dependent activation of NF-kappaB RelA/p50 heterodimer. J. Biol. Chem. 2012; 287:11924-33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7559

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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