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Western blot analysis in A375 cell line lysates (35ug/lane).
Western blot analysis in A375 cell line lysates (35ug/lane).

DPH1 Antibody

CATALOG NUMBER: 58-570

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-412 amino acids from the C-terminal region of human DPH1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DPH1
Additional Names:
Diphthamide biosynthesis protein 1, DPH1 homolog, HsDph1, Diphthamide biosynthesis protein 2 homolog-like 1, DPH-like 1, DPH2-like 1, Diphthamide biosynthesis protein 2-like, Ovarian cancer-associated gene 1 protein, DPH1, DPH2L, DPH2L1, OVCA1
Protein Accession Number:
Q9BZG8
Protein Gi Number:
269849559
Ncbi Gene Id Number:
1801
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Diphthamide is a unique posttranslationally modifiedhistidine found only in translation elongation factor-2 (EEF2; MIM130610). This modification is conserved from archaebacteria tohumans and serves as the target for ADP-ribosylation andinactivation of EEF2 by diphtheria toxin (DT) and Pseudomonasexotoxin A. DPH1 is 1 of several enzymes involved in synthesis ofdiphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[suppliedby OMIM].
Background Reference 1:
Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497(2004)
Background Reference 2:
Cardoso, C., et al. Am. J. Hum. Genet. 72(4):918-930(2003)
Background Reference 3:
Chen, C.M., et al. Biochem. Biophys. Res. Commun. 286(5):1019-1026(2001)
Background Reference 4:
Salicioni, A.M., et al. Genomics 69(1):54-62(2000)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-570

List Size:
400 ul

List Price:

$495.00

Datasheet
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