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Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Antibody used in WB on Human 293T at 0.2-1 ug/ml.

DPH2 Antibody

CATALOG NUMBER: 26-626

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DPH2 antibody can be used for detection of DPH2 by ELISA at 1:62500. DPH2 antibody can be used for detection of DPH2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
293T Cell Lysate
Predicted Molecular Weight:
52 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DPH2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DPH2
Additional Names:
DPH2, DPH2L2
Protein Accession Number:
NP_001375
Protein Gi Number:
21536476
Ncbi Gene Id Number:
1802
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DPH2 gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-626

List Size:
100 ul

List Price:

$519.00

Datasheet
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