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Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.

DPH2 Antibody

CATALOG NUMBER: 30-786

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
DPH2 antibody can be used for detection of DPH2 by ELISA at 1:1562500. DPH2 antibody can be used for detection of DPH2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
52 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store DPH2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
DPH2
Additional Names:
DPH2, DPH2L2
Protein Accession Number:
NP_001375
Protein Gi Number:
21536476
Ncbi Gene Id Number:
1802
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-786

List Size:
100 ul

List Price:

$519.00

Datasheet
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